Xinghao Li , Jia Xuan

Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Bioscience Methods, 2024, Vol. 15, No. 3   doi: 10.5376/bm.2024.15.0011
Received: 26 Mar., 2024    Accepted: 21 May, 2024    Published: 31 May, 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li X.H., and Xuan J., 2024, Molecular diagnostics: a new era in pet disease detection, Bioscience Method, 15(3): 91-101 (doi: 10.5376/bm.2024.15.0011)

The field of pet disease detection has traditionally relied on conventional diagnostic methods, which, while effective, often lack the sensitivity and specificity required for early and accurate disease identification. The emergence of molecular diagnostics has revolutionized this landscape, offering precise, rapid, and comprehensive tools for detecting a wide range of diseases in pets. This study explores the fundamentals of molecular diagnostics, including key concepts and techniques such as Polymerase Chain Reaction (PCR), Next-Generation Sequencing (NGS), microarrays, and CRISPR-based diagnostics, highlighting their advantages over traditional methods. We examine the applications of these technologies in the detection of infectious diseases, genetic disorders, oncology, and chronic disease management in pets. The study also delves into recent technological advancements, including improvements in PCR technology, innovations in sequencing platforms, the integration of artificial intelligence, and the development of portable diagnostic tools. Despite the significant promise of molecular diagnostics, challenges such as technical limitations, cost, accessibility, ethical concerns, and regulatory issues remain. A detailed case study illustrates the practical application of these diagnostics in a real-world veterinary scenario, offering insights into outcomes and future directions. Finally, we discuss the future potential of molecular diagnostics in personalized veterinary medicine, its integration with telemedicine, and its role in preventive care. This study underscores the transformative impact of molecular diagnostics on veterinary practice and calls for further research to overcome existing challenges and fully realize its potential.

Molecular diagnostics; Pet disease detection; Veterinary oncology; Genetic screening; Preventive veterinary medicine